Characterization and sequence of a Thermomonospora fusca xylanase
نویسندگان
چکیده
منابع مشابه
Cloning of a Thermomonospora fusca xylanase gene and its expression in Escherichia coli and Streptomyces lividans.
Thermomonospora fusca chromosomal DNA was partially digested with EcoRI to obtain 4- to 14-kilobase fragments, which were used to construct a library of recombinant phage by ligation with EcoRI arms of lambda gtWES. lambda B. A recombinant phage coding for xylanase activity which contained a 14-kilobase insert was identified. The xylanase gene was localized to a 2.1-kilobase SalI fragment of th...
متن کاملCharacterization of Endoxylanase Activity From Thermomonospora Fusca BD25
Thermomonospora fusca BD25 produces relatively high levels of activity of endoxylanases. Only oat spelt xylan, RBB-xylan and birchwood xylan reacted with endoxylanases with the maximum specific activity of 47.65 U mg-1 protein on birchwood xylan. The endoxylanase remained stable up to 70 °C. At 60 °C endoxylanase activity showed stability (92 %) for 16 h, however it showed a half-life of 8 h at...
متن کاملCellulases of Thermomonospora fusca and Streptomyces thermodiastaticus.
The cellulases of Streptomyces thermodiastaticus (strain 2Sts) and thermomonospora fusca (strain 190Th) were produced with carboxymethyl-cellulose (CMC) serving as the carbon source during growth. Both cellulases act by random internal hydrolysis of the CMC chain, producing cellobiose, glucose, and intermediate length oligosaccharides. Cellobiase was not detected in culture filtrates produced u...
متن کاملCharacterization and cloning of celR, a transcriptional regulator of cellulase genes from Thermomonospora fusca.
CelR, a protein that regulates transcription of cellulase genes in Thermomonospora fusca (Actinomycetaceae) was purified to homogeneity. A 6-kilobase NotI-SacI fragment of T. fusca DNA containing the celR gene was cloned into Esherichia coli and sequenced. The celR gene encodes a 340-residue polypeptide that is highly homologous to members of the GalR-LacI family of bacterial transcriptional re...
متن کاملGenetic Recombination and Transformation in Protoplasts of Thermomonospora fusca.
Protoplasts were produced from the thermophilic actinomycete Thermomonospora fusca and were regenerated to 0.1% of the direct count on regeneration agar. Recombination after protoplast fusion was demonstrated with drug-resistant mutants of T. fusca YX. A single thiostrepton-resistant colony was isolated after transformation of T. fusca YX with the streptomycete vector pIJ702, providing the firs...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1994
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.60.3.763-770.1994